Western blot analysis of C1QA in RPMI 8226 cells. Samples were incubated with C1QA Polyclonal antibody (Product # PA5-95941) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot analysis of C1QA in extracts of various cell lines using C1QA Polyclonal Antibody (Product # PA5-95941) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supplier Page from Thermo Fisher Scientific for C1QA Antibody